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Determination of polyaromatic hydrocarbons (PAH'S) in fresh processed and cooked fishes by S. Kannappan

By: Kannappan | [Researcher]Material type: TextTextPublication details: Thoothukudi ; Fisheries College and Research Institute ; 1993 Edition: M. F. ScDescription: 72pSubject(s): Polyaromatic hydrocarbon - Processed fishDDC classification: 664
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"Different fresh fish purchased from Tuticorin fish market, were made into various products viz. curry, cooked, deep fat fried, broiled and grilled. Samples from these were extracted for determination of polyaromatic hydrocarbons (PAH's) particularly chrysene, benzo(a)pyrene and dibenzanthracene using different organic solvents and evaporated in a vacuum evaporator at 60�C. They were dissolved in acetonitrile and analyzed for PAH content by High Performance Liquid Chromatography (HPLC). Authentic standards of chrysene, dibenzanthracene and benzo(a)pyrene (Sigma USA) were used for identification. A known aliquot of the sample was injected into shimadzu (HPLC LC6A JAPAN) shim-pack CLC - ODS (6.0mm i.dx 15 cm) column at 35�C column temperature at the flow rate 1ml/min. The values were measured at 254 nm with 0.005 absorbance. Hundred percent acetonitrile was used as mobile phase and the samples were identified and quantified under ternary gradient. Detectable quantities of PAH's have been found in samples of processed and cooked fish. Chrysene a mild carcinogen was present in majority of the samples analyzed, followed by dibenzanthracene. Benzo(a)pyrene was present only in broiled, grilled, shallow fat fried samples and salt dried fishery products."

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